Monte Rosa Therapeutics is seeking a passionate Scientist with extensive experience in biochemical, biophysical, and protein purification assays. In this role, you will design, develop, and execute kinetic-focused assays and advanced protein purification protocols—including those for challenging protein targets—using a range of techniques. Experience with in vitro transcription translation (IVTT) systems is a plus. This position is a key contributor to the characterization of novel small molecule glue-based degraders, supporting Monte Rosa’s growing pipeline. The candidate should exhibit excellent communication and collaborative skills for working on cross-functional teams, as well as demonstrate a high degree of independence and organization.

Protein Biochemistry & Purification:
• Develop and optimize protein expression and purification protocols, including strategies for difficult or challenging targets (e.g., proteins with solubility issues, aggregation, or instability) in bacterial and mammalian expression system.
• Apply advanced protein purification techniques such as affinity chromatography, ion exchange, size exclusion chromatography, and other specialized methods.
• Implement in vitro transcription translation (IVTT) systems for rapid protein expression screening and method development.
• Troubleshoot and refine purification processes to maximize protein yield, purity, and functional integrity.

Biochemical & Biophysical Assay Development:
• Design, develop and implement a variety of kinetic-focused, protein-protein interaction assays in low and high-throughput settings.
o Standard assays include surface plasmon resonance (SPR), MST, Spectral Shift, Differential Scanning Fluorimetry (DSF), Flow-induced dispersion analysis (FIDA), TR-FRET, and fluorescence polarization (FP).
• Collaborate with interdisciplinary teams to integrate biochemical, biophysical, and protein purification data in support of drug discovery initiatives
• Screen and characterize small molecule compounds and determine the mode of action.
• Analyze and interpret integrated datasets.
• Maintain laboratory records, protocols, and summaries.
• Contribute to laboratory organization and maintenance.
• Present research plans and results, both within the team and to senior management.

• PhD: In Biochemistry, Biophysics, Molecular Biology, or a related field with a minimum of 2 years of industry experience in a pharma/biotech drug discovery environment;
• MS: In Biochemistry, Biophysics, Molecular Biology, or a related field with 4–6 years of industry experience.

Technical Skills:

• Strong background in protein biochemistry with demonstrated experience in purifying and characterizing protein targets. Hands-on experience with IVTT systems for protein expression and screening is a plus.
• Strong background in biochemical and/or biophysical assays (e.g., (fluorescence, TR-FRET, FP, MST, SPR, and/or DSF) is a plus.
• Skilled in advanced protein purification and characterization techniques, including affinity, ion exchange, and size exclusion chromatography, Western Blotting, mass spectrometry is a plus.
• Experience with various compound screening formats, laboratory instrumentation, and high throughput automation is a plus.
• Proven ability to work independently and design experiments, troubleshoot, interpret and report large datasets.
• Highly analytical mind, attention to detail and organization skills are critical.